Stability of housekeeping genes in alveolar macrophages from COPD patients
نویسندگان
چکیده
منابع مشابه
Stability of housekeeping genes in alveolar macrophages from COPD patients.
The stability of housekeeping genes is critical when performing gene expression studies. To date, there have been no studies that look at the stability of commonly used housekeeping genes in alveolar macrophages. Expression levels may be affected by culture, stimulation or disease severity. The present study investigated the expression level of 10 housekeeping genes and analysed the stability o...
متن کاملTelomere Shortening in Alveolar Macrophages of Smokers and COPD Patients
Background: Tobacco smoking is reported to enhance telomere shortening in peripheral blood lymphocytes. However, telomere length of lung resident cells such as alveolar macrophages (AMs) has not been evaluated. Aim: We evaluated effect of tobacco smoking on telomere length in AMs. Methods: AMs were obtained from nine non-smokers (mean age 24.6 years, mean FEV1/FVC 93.8%) and ten healthy smokers...
متن کاملCHROMOSOMAL PROTEINS IN PULMONARY ALVEOLAR MACROPHAGES
In this study the nature of chromosomal proteins, his tones and nonhistone in resident alveolar macrophages was investigated in comparison to peritoneal neutrophils and calf thymus proteins. Cells were obtained by lavaging the lung and after purity determination they were subjected to fractional extraction procedures. Proteins were then analysed on SDS polyacrylamide gels and densitometric...
متن کاملImpaired Inhibition by Dexamethasone of Cytokine Release by Alveolar Macrophages from COPD Patients
Chronic obstructive pulmonary disease (COPD) is characterized by inflammation of the respiratory tract in which macrophages are the predominant inflammatory cell and for which the efficacy of treatment with corticosteroids is controversial. We investigated the effect of dexamethasone on basal and interleukin (IL)-1β or cigarette smoke media (CSM) stimulated release of IL-8 and granulocyte macro...
متن کاملApoptosis of circulating neutrophils and alveolar macrophages in COPD.
vs interferon (IFN)showed that IFNis superior to ADA, although the difference is small. Therefore, establishing a diagnosis of tuberculosis pleuritis based only on pleural fluid ADA without pleural biopsy findings is still controversial.6 We do not intend to deny the usefulness of pleural fluid ADA for diagnosing tuberculosis pleuritis. Furthermore, we do not insist that pleural fluid INFcan re...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: European Respiratory Journal
سال: 2006
ISSN: 0903-1936,1399-3003
DOI: 10.1183/09031936.06.00090405